Compatibility of Coglapest® and Coglapix® vaccines in pigs

This study was conducted in order to test the compatibility of Coglapix® and Coglapest® vaccines, when the latter was reconstituted in Coglapix®.




--> Introduction

--> Materials and Methods

--> Results

--> Discussion and Conclusions




By Vilmos Palya1, István Kiss1, Roman Krejci2

1Ceva Sante Animale, Scientific Support and Investigation Unit, Ceva-Phylaxia Ltd., Budapest, Hungary,
2Ceva, Libourne, France.



Porcine pleuropneumonia is a highly contagious respiratory disease caused by Actinobacillus pleuropneumoniae (A.p.). The disease occurs worldwide with an increasing incidence. Controlling the disease presents a challenge, for its rapid onset and persistence in infected herds, and because of the issue of antibiotic resistance. Vaccination, however, can provide efficient protection against the disease by decreasing the prevalence and extension of pneumonia and pleuritis and as a result animals with better weight can be delivered to the market.

Classical swine fever (CSF) is a highly contagious disease of pigs with the potential of devastating epidemics, which also occurs widely in some parts of the world. Outbreaks of CSF are controlled by strict measures, such as culling and movement restrictions, which, besides the direct consequences of the disease, have severe impact on the pig industry. In countries where the virus is endemic, vaccination is used to prevent the spread of the virus and the consequence of infection.

Based on practical and economic considerations the simultaneous vaccination of piglets against several diseases is desired, provided that the efficacy of each vaccine is not compromised. Upon the demand of vet practitioners the study was conducted in order to test the compatibility of Coglapix® and Coglapest® vaccines, when the latter was reconstituted in Coglapix®.

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Materials and Methods

Six weeks old pigs were vaccinated twice, three weeks apart, either with Coglapest® or Coglapix® or combined vaccine of Coglapest® and Coglapix®. The efficacy of Coglapix® was tested by challenging the pigs with an A.p. serotype-2 strain via aerosol in a dedicated chamber by applying approximately 108 CCU/pig of the bacterium suspension. Parameters related to immunity (humoral immune response and protection against A.p. challenge) were compared between the vaccinated and control pigs.

APX-2 ELISA (in-house assay) and Chekit APX-4 ELISA (Idexx) was used to measure antibody response to Coglapix® and neutralising peroxidase-linked assay (NPLA) was used to measure antibody response to Coglapest®.

Scoring of lung lesions was performed according to Hannah et al., 1982., and the efficacy of Coglapix® vaccine was calculated according to Jones et al., 2005.

Serological results, body weight gain, and lung lesion score data were analysed by ANOVA. Differences were considered significant at p<0.05.

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Humoral immune response to Coglapest® did not differ significantly between the Coglapest®/Coglapix® combined and Coglapest® alone vaccinated groups (Figure 1).

Figure 1 Box-and-Whisker plot of antibody response against CSFV

Following challenge with A.p. serotype-2 strain, 20% of the unvaccinated control pigs died, while no mortality in the vaccinated groups was recorded. The two vaccinated groups had significantly higher mean body weight gain than the unvaccinated control group, but did not differ significantly from each other. Further, the mean pathological scores were significantly higher for the non-vaccinated control group than for the two vaccinated groups, which did not differ significantly (Figure 2).

Figure 2. Box-and-Whisker plot of the lung lesion score data of the groups following A.p. serotype 2 challenge.

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Discussion and Conclusions

Pigs vaccinated with Coglapest® either alone or in combination with Coglapix® equally seroconverted and produced very similar amount of CSFV VN antibodies both after the primary and also after the booster vaccination.

Vaccination with Coglapix® either alone or in combination with Coglapest® induced high level of protection against the challenge with the virulent strain of A.p. serotype 2 without significant difference between the two vaccination regimens.

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Jones, G.F., et al., J. Swine Health Prod. 2005; 13(1): 19–27.

Hannan, P., et al., Res. Vet. Sci. 1982. 33: 76-88.


(Source: Axis Issue 02 / Sept 2013 - Ceva Asia Pacific - Proceedings APVS 2013)


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